(Eosin methylene blue compound)  Direction for preparation of reagent:  Leishman’s stain powder 0.15g Methanol GR 100 ml (Dissolve powder in methanol and allow to stand for 24 hrs. and filter.) (Staining of blood smears and connective tissues and papanicolaou’s staining) Absorption Max (MeOH) l1 max640-645 nm Absorption Max (MeOH l2 max 522-525nm Absorptivity (A1%/1cm in MeOH, atl1 max On dried substance) min 900 Absorptivity (A1%/1cm in MeOH, at l2 max on dried substance) min 650 LOD (110OC) min 8.0% Suitability for microscopy passes test